The Comparative Study of the Mouse Osteoblast Response to Two Different Platelet-rich Fibrins with Low Speed Centrifugation Concept

Objectives: To compare the response of mouse osteoblast (MC3T3-E1 cells) to advanced platelet-rich fibrin (APRF) and advanced platelet-rich fibrin plus (APRF+).

Methods: Blood was collected from eight volunteers, 25 - 38 years of age (four males and four females) to prepare platelet concentrates APRF (1,300 rpm, 14 minutes), APRF+ (1,300 rpm, 8 minutes). The exudates were collected from both platelet concentrates at day 1, 3, 7 and 14. The level of TGF-β1 from exudates were quantified using an ELISA. MC3T3-E1 cells were cultured with the exudates. The cultured cells were tested with MTT assays, alkaline phosphatase (ALP) staining and mineralization, which were analyzed on day 7 and again on day 14.

Results: APRF and APRF+ continuously released TGF-β1 during 14-days period. Only exudates collected at day 1 showed significantly difference of ALP staining between APRF and APRF+ group on day 14 of observation. On day 7 of mineralization assays, cells treated with exudates from APRF+ collected at day 14 resulted in the highest level of mineralization within APRF+ group. On day 14 of mineralization assays, cells treated with exudates from APRF collected at day 7 showed the highest level of mineralization within APRF group.

Conclusions: APRF+ released TGF-β1 at day 14 significantly higher than day 1. On day 14 of mineralization, cells treated with exudates from APRF+ collected at day 14 showed significantly higher mineralization than APRF collected at the same time point.

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Domprapagon P, Tachasuttirut K, Permpanich P. The Comparative Study of the Mouse Osteoblast Response to Two Different Platelet-rich Fibrins with Low Speed Centrifugation Concept: Original articles. CM Dent J [Internet]. 2024 Apr 18 [cited 2024 Jul 15];45(1):39-46. Available from: https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=213

Domprapagon, P., Tachasuttirut, K. & Permpanich, P. (2024). The Comparative Study of the Mouse Osteoblast Response to Two Different Platelet-rich Fibrins with Low Speed Centrifugation Concept. CM Dent J, 45(1), 39-46. Retrieved from: https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=213

Domprapagon, P., Tachasuttirut Kathawut and Permpanich Piyanuj. 2024. "The Comparative Study of the Mouse Osteoblast Response to Two Different Platelet-rich Fibrins with Low Speed Centrifugation Concept." CM Dent J, 45(1), 39-46. https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=213

Domprapagon, P. et al. 2024. 'The Comparative Study of the Mouse Osteoblast Response to Two Different Platelet-rich Fibrins with Low Speed Centrifugation Concept', CM Dent J, 45(1), 39-46. Retrieved from https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=213

Domprapagon, P., Tachasuttirut, K. and Permpanich, P. "The Comparative Study of the Mouse Osteoblast Response to Two Different Platelet-rich Fibrins with Low Speed Centrifugation Concept", CM Dent J, vol.45, no. 1, pp. 39-46, Apr. 2024.

Domprapagon Patthapong, Tachasuttirut Kathawut, Permpanich Piyanuj "The Comparative Study of the Mouse Osteoblast Response to Two Different Platelet-rich Fibrins with Low Speed Centrifugation Concept." CM Dent J, vol.45, no. 1, Apr. 2024, pp. 39-46, https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=213