Efficiency of Disinfection with Sodium Hypochlorite, Glutaraldehyde and Peracetic Acid on Irreversible Hydrocolloid Impression
The major problem of disinfecting the hydrocolloid impression is the microorganisms that can be imbibed into the impression. The efficient of disinfection depends on disinfectant type and method with sufficient length of treatment time. The purpose of this study was to test the efficacy of 3 disinfectants: sodium hypochlorite, glutaraldehyde, peracetic acid by spray and immersion the irreversible hydrocolloid impression for 5 and 10 minutes. The microorganisms used in this study were Pseudomonas aeruginosa and Candida albicans. Four specimens of contaminated irreversible hydrocolloid in each group were treated by 3 disinfectants by 4 methods: spray 5 and 10 minutes and immersion 5 and 10 minutes. The control group was treated by distilled water. The result was measured by counting the colonies of microorganisms left from disinfection after inoculated and incubated for 24 hours. It was found that the efficacies of all 3 disinfectants were able to inactivate both microorganisms. Both methods of treatment by spray and immersion with 5 and 10 minutes were able to reduce microorganisms statistically significant compared with control group.
1. Owen CP, Goolam R. Disinfection of impression materials to prevent viral cross contamination: A review and a protocol. Int J Prosthodont 1993; 6: 480-494.
2. Watkinson AC. Disinfection of impressions in UK Dental Schools. Br Dent J 1988; 164: 22- 23.
3. Molinari JA, Runnells RR. Role of disinfectants in infection control. Dent Clin North Am 1991; 35: 323-337.
4. ADA Council on Scientific Affairs and ADA Council on Dental Practice. Infection control recommendations for the dental office and the dental laboratory. J Am Dent Assoc 1996; 127: 672-680.
5. Gardner JF, Peel MM. Introduction to Sterilization, Disinfection and Infection Control. 2nd ed. London, Churchill Livingstone; 1991: 151-161.
6. Look JO, Clay DJ, Gong K, Messer HH. Preliminary results from disinfection of irreversible hydrocolloid impressions. J Prosthet Dent 1990; 63: 701-707.
7. Jennings KJ, Samaranayake LP. The persistence of microorganisms on impression materials following disinfection. Int J Prosthodont 1991; 4: 382-387.
8. Merchant VA. Infection control and prosthodontics. J Calif Dent Assoc 1989; 17: 49- 53.
9. Rueggebery FA, Beall FE, Kelly MT, Schuster GS. Sodium hypochlorite disinfection of irreversible hydrocolloid impression material. J Prosthet Dent 1992; 67: 628-631.
10. Westerholm HS, Bradley DV, Schwartz RS. Efficacy of various spray disinfectants on irreversible hydrocolloid impressions. Int J Prosthodont 1992; 5: 47-54.
11. Beyerle MP, Hensley DM, Bradley DV, Schwartz RS, Hilton TJ. Immersion disinfection of irreversible hydrocolloid impressions with sodium hypochlorite. Part I: Microbiology. Int J Prosthodont 1994; 7: 234- 238.
12. Infection control recommendations for the dental office and the dental laboratory. Council on Dental Materials, Instruments, and Equipment. Council on Dental Practice. Council on Dental Therapeutics. J Am Dent Assoc 1988; 116:241-248.
13. Tan H, Wolfaardt JF, Hooper PM, Busby B. Effects of disinfecting irreversible hydrocolloid impressions on the resultant gypsum casts: Part I-Surface quality. J Prosthet Dent 1993; 69: 250-257.
14. Durr DP, Novak EV. Dimensional stability of alginate immersed in disinfecting solutions. J Dent Child 1987; 54: 45-48.
15. Johnson GH, Chellis KD, Gordon GE, Lepe X. Dimensional stability and detail reproduction of irreversible hydrocolloid and elastomeric impressions disinfected by immersion. J Prosthet Dent 1998; 79: 446-453.
16. Hilton TJ, Schwartz RS, Bradley DV. Immersion disinfection of irreversible hydrocolloid impressions. Part 2: Effects on gypsum casts. Int J Prosthodont 1994; 7: 424-33.
17. Vandewalle KS, Charlton DG, Schwartz RS, Reagan SE, Koeppen RG. Immersion disinfection of irreversible hydrocolloid impressions with sodium hypochlorite. Part II: Effect on Gypsum. Int J Prosthodont 1994; 7:315- 322.