Forensic Identification by Polymerase Chain Reaction of DNA Extracted from Primary Teeth with Dental Caries
Objectives: The aim of this research is to determine the effect of restoration time of deciduous teeth on human identification efficiency using microsatellite DNA
Methods: Using human genomic DNAs were isolated from deciduous molar in Thai children (n=26) under 12 years and were compared with DNA from the blood in sterile gauze pads. All samples were divided into 2 groups of 13 teeth, which were kept at room temperature for a period of 1 day and 1 month, respectively. Tooth DNAs were extracted using QIAamp® DNA Investigator and then microsatellite DNA on D3S1358, D5S818 and D16S539 loci were amplified using polymerase chain reaction.
Results: DNA could be detected from 2 groups. Microsatellite DNA on D3S1358, D5S818 and D16S539 loci showed the same results as the blood samples.
Conclusions: Deciduous teeth stored at room temperature for one month had no affected on human identification efficiency using microsatellite DNA.
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