The Collagen Fibers Analysis of the Odontogenic Cysts: A Study with Picrosirius Red Staining Under Polarizing Microscopy

Udompatanakorn Chatchaphan, Rungsiyanont Sorasun

Published Online: Jan 27, 2022 DOI 10.14456/cmdj.2022.4

Objectives: This study aims to compare the polarization colors of the collagen fibers in the connective tissue wall (CNT) of radicular cyst (RC), dentigerous cyst (DC), odontogenic keratocyst (OKC), and calcifying odontogenic cyst (COC).

Material and methods: Collagen in the CNT of ten patients diagnosed with RC, DC, OKC, or COC was stained with picrosirius red staining and examined under a polarizing microscope. The birefringence of collagen fibers of odontogenic cysts (OCs) regarding the frequency and the labeling index (LI) scores based on the proportion of mature collagen (orange-red fibers) and immature collagen (greenish-yellow fibers) were compared.

Results: The orange-red polarization color was observed predominantly in the CNT of DCs (90.0%), RCs (70.0%), and OKCs (60.0%). Meanwhile, the greenish-yellow polarization color predominated in the CNT of COCs samples (50.0%). The mean LI values of RCs, DCs, OKCs, and COCs were 1.93±0.73, 1.90±0.44, 2.03±0.93, and 1.40±0.59, respectively. There is no statistically significant difference between the groups (p>0.05).

Conclusions: Although no statistically significant difference between OCs was observed, the collagen fibers of COCs were different from other OCs. The greenish-yellow polarization color predominantly observed in COCs suggested that the CNTs of some COCs might play a role in the cystic neoplasm behavior.

picrosirius red staining collagen odontogenic cyst polarizing microscopy

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Udompatanakorn C, Rungsiyanont S. The Collagen Fibers Analysis of the Odontogenic Cysts: A Study with Picrosirius Red Staining Under Polarizing Microscopy: Original articles. CM Dent J [Internet]. 2022 Jan 27 [cited 2024 Apr 29];43(1):39-48. Available from: https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=160

Udompatanakorn, C. & Rungsiyanont, S. (2022). The Collagen Fibers Analysis of the Odontogenic Cysts: A Study with Picrosirius Red Staining Under Polarizing Microscopy. CM Dent J, 43(1), 39-48. Retrieved from: https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=160

Udompatanakorn, C., and Rungsiyanont Sorasun. 2022. "The Collagen Fibers Analysis of the Odontogenic Cysts: A Study with Picrosirius Red Staining Under Polarizing Microscopy." CM Dent J, 43(1), 39-48. https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=160

Udompatanakorn, C. et al. 2022. 'The Collagen Fibers Analysis of the Odontogenic Cysts: A Study with Picrosirius Red Staining Under Polarizing Microscopy', CM Dent J, 43(1), 39-48. Retrieved from https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=160

Udompatanakorn, C. and Rungsiyanont, S. "The Collagen Fibers Analysis of the Odontogenic Cysts: A Study with Picrosirius Red Staining Under Polarizing Microscopy", CM Dent J, vol.43, no. 1, pp. 39-48, Jan. 2022.

Udompatanakorn Chatchaphan, Rungsiyanont Sorasun "The Collagen Fibers Analysis of the Odontogenic Cysts: A Study with Picrosirius Red Staining Under Polarizing Microscopy." CM Dent J, vol.43, no. 1, Jan. 2022, pp. 39-48, https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=160