Raised Chondroitin Sulphate (WF6 epitope) Levels around Maxillary Molars and Miniscrew Implants during Orthodontic Molar Intrusion
This study aimed to monitor chondroitin sulphate (CS; WF6 epitope) levels in crevicular fluid around maxillary molars and miniscrew implants during orthodontic molar intrusion. One miniscrew implant was placed in the midpalatal area of each of ten patients with open skeletal configurations, who required orthodontic molar intrusion, and two Sentalloy® closed-coil springs (100 g) were used for molar intrusion. Gingival crevicular fluid (GCF) around experimental and control molars, and peri-miniscrew implant crevicular fluid (PMICF) were collected before and during load application. Competitive ELISA with monoclonal antibody WF6 and colorimetric protein assay were used to detect CS (WF6 epitope), and total protein concentration, respectively. The results showed that the median CS (WF6 epitope) levels around experimental molars during the loaded period (12 weeks) (2.099 ng/μg of total protein) and those during each two-week interval of the loaded period (1.952, 1.854, 2.604, 2.414, 1.844, 1.44 ng/ μg of total protein respectively) were significantly greater than those during the unloaded period (2 weeks) (0.832 ng/ μg of total protein) (P<0.05), whereas the median CS (WF6 epitope) levels around control molars and around miniscrew implants, during the unloaded and loaded periods, were not significantly different. The results of this study may emphasize the role of CS (WF6 epitope) level as a biomarker for alveolar bone resorption around orthodontically moved teeth and also around miniscrew implants.
1. Yoa CCJ, Lee JJ, Chang ZCJ, Chang HF, Chen YJ. Maxillary molar intrusion with fixed appliances and mini-implant anchorage studied in three Dimensions. Angle Orthod 2005; 75(5):754-60.
2. Xun C, Zeng X, Wang X. Microscrew anchorage in skeletal anterior open-bite treatment. Angle Orthod 2007; 77(1):47-56.
3. Park HS, Kwon TG, Kwon OW. Treatment of open bite with microscrew implant anchorage. Am J Orthod Dentofacial Orthop 2004; 126(5):627-36.
4. Park HS, Jang BK, Kyung HM. Maxillary molar intrusion with micro-implant anchorage (MIA). Aust Orthod J 2005; 21(2):129-35.
5. Lee JS, Kim DH, Park YC, Kyung SH, Kim TK. The efficient use of midpalatal miniscrew implants. Angle Orthod 2004; 74(5):711-14.
6. Dudic A, Kiliaridis S, Mombelli A, Giannopoulou C. Composition changes in gingival crevicular fluid during orthodontic tooth movement: comparisons between tension and compression sides. Eur J Oral Sci 2006; 114:416-22.
7. Waddington RJ, Embery G, Samuels RH. Characterization of proteoglycan metabolites in human gingival crevicular fluid during orthodontic tooth movement. Arch Oral Biol 1994; 39:361-68.
8. Khongkhunthian S, Srimueang N, Krisanaprakornkit S, Pattanaporn K, Ong-Chia S, Kongtawelert P. Raise chondroitin sulphate WF6 epitope levels in gingival crevicular fluid in chronic periodontitis. J Clin Periodontol 2008; 35:871-76.
9. Last KS, Donkin C, Embery G. Glycosaminoglycans in human gingival crevicular fluid during orthodontic movement. Arch Oral Biol 1988; 33:907-12.
10. Jaito N, Jotikasthira D, Krisanaprakornkit S, Ong-chai S, Kongtawelert P. Monitoring of chondroitin-6-sulfate levels in gingival crevicular fluid during orthodontic canine movement. In: Davidovitch Z MJ, Suthanarak S, editor. Biological Mechanisms of Tooth Eruption, Resorption and Movement. Boston: Harvard Society for the Advancement of Orthodontics, Boston, Massachusetts; 2006. p. 289-95.
11. Intachai I, Kritsanaprakornkit S, Kongtawelert P, Ong-chai S, Buranastidporn B, Suzuki EY, Jotikasthira D. Chondroitin sulphate epitope (WF6 epitope) levels in peri-miniscrew implant crevicular fluid during orthodontic loading. Eur J Orthod 2010; 32:60-65.
12. Shibutani Y, Nishino W, Shiraki M, Iwayama Y. ELISA detection of glycosaminoglycan (GAG)-linked proteoglycans in gingival crevicular fluid. J Periodont Res 1993; 28:17- 20.
13. Park HS, Jeong SH, Kwon OW. Factors affecting the clinical success of screw implants used as orthodontic anchorage. Am J Orthod Dentofacial Orthop 2006; 130:18-25.
14. Last KS, Stanbury JB, Embrey G. Glycosaminoglycans in human gingival crevicular fluid as indicators of active periodontal disease. Arch Oral Biol 1985; 30:275-81.
15. Last KS, Smith S, Pender N. Monitoring of IMZ titanium endosseous dental implants by glycosaminoglycan analysis of peri-implant sulcus fluid. Int J Oral Maxillofac Implants 1995; 10:58-66.
16. Okazaki J, Gonda Y, Kamada A, Sakaki T, Kitayama N, Kawamura T, Ueda M. Disaccharide analysis of chondroitin sulfate in periimplant sulcus fluid from dental implants. Eur J Oral Sci 1996; 104:141-43.
17. Beck CB, Embrey G, Langley MS, Waddington RJ. Levels of glycosaminiglycans in prei-implant sulcus fluid as a means of monitoring bone response to endosseous dental implants. Clin Oral Implants Res 1991; 2:179-85.
18. Smedberg JI, Beck CB, Embrey G. Glycosaminoglycans in peri-implant sulcus fluid from implants supporting fixed or removable prosthesis. Clin Oral Implants Res 1993; 4:137-43.
19. Samuels RHA, Pender N, Last KS. The effects of orthodontic tooth movement on the glycosaminoglycan components of gingival crevicular fluid. J Clin Periodontol 1993; 20:371-77.
20. Baldwin PD, Pender N, Last KS. Effects on tooth movement of force delivery from nickeltitanium archwires. Eur J Orthod 1999; 21: 481-89.
21. Kagayama M, Samano y, Mizoguchi I, Kamo N, Takahashi I, Mitani H. Localization of glycosaminoglycans in periodontal ligament during physiological and experimental tooth movement. J Periodont Res 1996; 31:229-34.
22. Faltin RM, Faltin K, Sander FG, AranaChavez VE. Ultrastructure of cementum and periodontal ligament after continuous intrusion in humans: a transmission electron microscopy study. Eur J Orthod 2001; 23:35- 49.
23. Embery G, Oliver W, Stanbury JB, Purvis J A. The electrophoretic detection of acid glycosminoglycans in human gingival sulcus fluid. Arch Oral Biol 1982; 27:177-79.