Antimicrobial Activity of Curcuma longa Linn. Crude Extract Against Enterococcus faecalis Planktonic and Biofi lm Growth
The aim of this study was to determine the antibacterial activity of Curcuma longa Linn. Crude extract that against Enterococcus faecalis planktonic and biofilm.
Method: Crude extracts from Curcuma were extracted by 95% ethanol. The antibacterial activity against Enterococcus faecalis was carried out by agar disc diffusion assay and determined both MIC and MBC of crude extracts against Enterococcus faecalis planktonic and biofilm by broth dilution assay compared to chlorhexidine gluconate (0.2 % w/v).
Result: The MIC and MBC of the crude extracts against Enterococcus faecalis planktonic were 25 mg/ml and 50 mg/ml, respectively, whereas those of 0.2% chlorhexidine gluconate were 0.031 mg/ml and 0.063 mg/ml, respectively. The crude extract was able to totally inhibit growth of Enterococcuc faecalis biofilms at concentration of 300 mg/ml. In addition, the crude extracts inhibited Enterococcus faecalis established biofilm at the minimal concentration of 37.5 mg/ml, however the eradication of established Enterococcus faecalis biofilm was not seen at the highest concentration used in this study.
Conclusion: The crude extracts have antibacterial activity against Enterococcus faecalis both planktonic and biofilm. However, the concentration that can inhibit Enterococcus faecalis biofilm is higher than planktonic forms.
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