Age Estimation Using Pulp/Tooth Area Ratio of Upper Canines in a Thai Population

This study examined the correlation between age and pulp/tooth area ratio in maxillary canines in a Thai population, to develop an equation for age estimation and to test the accuracy of the equation. Sample of 544 periapical radiographs of maxillary canines were collected. The AutoCAD 2017 program was used in drawing the outline of pulp and tooth area and in measurement of the radiographs. Then, the pulp/tooth area ratio was calculated. A correlation coefficient (r) -0.475 (p<0.05) with linear regression was found using the Pearson correlation. The formula for age estimation was: Age = 78.895-270.614(AR), when AR is the pulp/ tooth area ratio of the maxillary canines. The coefficient of determination (R2) 0.225 and the standard error of estimation (SEE) was 13.664 years. Then, when the equation was tested in a test group (88 periapical radiographs) the mean error (ME) was -4.40 years and the mean absolute error (MAE) was 13.06 years. This is the first study using the pulp/tooth area ratio of maxillary canines for age estimation in Thailand. The equation developed in this study should be useful for age estimation in forensic anthropological and forensic science cases, particularly in Thai people over 20 years.

1. Pongpitak P. Age estimation by using cranial sutures and epiphyseal plate. J Forensic Med Department of Anatomy. Faculty of Medical Sciences. Naresuan University 2013; 4(3): 1-13. (in Thai)

2. Cameriere R, De Luca S, Aleman I, Ferrante L, Cingolani M. Age estimation by pulp/tooth ratio in lower premolar by orthopantomography. Forensic Sci Int 2012; 214: 105-112.

3. Priyadarshini C, Puranik MP, Uma SR. Dental age estimation method: A review. Int J Adv Health Sc 2015; 12(1): 19-25.

4. Cameriere R, De Luca S, Egidi N, et al. Automatic age estimation in adults by analysis of canine pulp/tooth ratio: Preliminary results. J Forensic Radio and Image 2015; 3: 61-66.

5. Kvaal SI, Kolltveit KM, Thomsen IO, Solheim T. Age estimation of adults from dental radiographs. Forensic Sci Int 1995; 74(1): 75-85.

6. Cameriere R, Ferrante L, Cingolani M. Variation in plup/tooth area ratio as an indicator of age: A preliminary study. J Forensic Sci 2004; 49(2): 317-319.

7. Cameriere R, Cunha E, Sassaroli E, Nuzzolese E, Ferrante L. Age estimation by pulp/tooth area ratio in canines: Study of a portuguese sample to test camerirer’s method. Forensic Sci Int 2009; 193: 1281-1286.

8. Cameriere R, Ferrante L, Belcastro MG, Bonfiglioli B, Rastelli E, Cingolani M. Age estimation by pulp/tooth ratio in canines by mesial and vestibular peri-apical x-ray. J Forensic Sci 2007; 52(5): 1151-1155.

9. Cameriere R, Ferrante L, Belcastro MG, Bonfiglioli B, Rastelli E, Cingolani M. Age estimation by pulp/tooth ratio in canines by peri-apical x-ray. J Forensic Sci 2007; 51(1): 166-170.

10. Cameriere R, Cunha E, Wasterlain S, et al. Age estimation by pulp/tooth ratio in lateral and central incisor by peri-apical x-ray. J Forensic Sci 2013; 20(5): 530-536.

11. Jeevan MB, Kale AD, Angadi PV, Hallikerimath S. Age estimation by pulp/tooth area ratio in canines: Cameriere's method assessed in an Indian sample using radiovisiography. J Forensic Sci 2011; 204(1-3): 209.e1-209.e5.

12. Juneja M, Devi YBK, Rakesh N, Juneja S. Age estimation using pulp/tooth area ratio in maxillary canines-A digital image analysis. J Dent Sci 2014; 6(3): 160-165.

13. Dar MA, Nayyar AS. A comparative analysis between various teeth in kvaal’s and cameriere’s method of age estimation in a specific populace of Andhra Pradesh: an original study. J Forensic Odontol 2016; 1 :26-35.

14. Hatice BD, Nihal A, Nursel A, Humeyra Ozge Y, Goksuluk D. Applicability of Cameriere’s and Drusini’s age estimation method to a sample of Turkish adults. Dentomaxillofac Radiol 2017; 46: 1-7.

15. Nazir N, Chalkoo AH. Age estimation from pulp/tooth ration by panoramic radiography: in Kashmiri population. J Maxillofac img 2017; 3(4): 115-119.

16. Dehghani M, Shandkam E, Ahrari F, Dehghani M. age estimation canine’s pulp/tooth ration in an Iranian population using digital panoramic radiography. Forensic Sci Int 2018; 285: 44-49.

17. Babshet M, Acharaya AB, Naikmasur VG. Age estimation in Indians from pulp/tooth area ratio of mandibular canines. J Forensic Sci. 2010; 197(1-3): 125.e1-125.e4.

18. Star H, Thevissen P, Fieuws S, Solheim T, Willems G. Human dental age estimation by calculation of pulp-tooth volume ratios yielded on clinically acquired cone beam computed tomography images of monoradicular teeth. J Forensic Sci 2011; 1: 77-82.

19. Sakhdari S, Mehralizadeh S, Zalfaghari M, Madadi M. Age estimation from pulp/tooth ratio using digital panoramic radiography. J of Islamic Dent Association of IRAN 2015; 27(1): 1-5.

20. Basoya S, VC V, Nath P, Bhogte AD, Estimation of age by pulp-tooth area ratio using three computer aided software’s. Quality In Primary Care 2016; 24(4): 161-166.

21. Yang F, Jacobs R, Willrms G. Dental age estimation through volume matching of teeth imaged by cone-beam CT. Forensic Sci Int 2006; 159(1): 78-83.

22. Angelis DD, Gaudio D, Guercini N, et al. Age estimation from canine volumes. Radiol Med 2015; 120: 731-736.

23. Ge ZP, Ma RH, Li G, Zhang JZ, Ma XC. Age estimation based on pulp chamber volume of first molars from cone-beam computed tomography images. Forensic Sci Int 2015; 253: 133.e1-133.e7.

24. Jagannathan N, Neelakantan P, Thriuvengadam C, et al. Age estimation in an indian population using pulp/tooth volume ratio of mandibular canines obtained from cone beam computed tomography. J Forensic Odontostomatol 2011; 29(1): 1-6.

25. Poommouang A, Lamaroon A, Prapayasatok S, Prasitwattanaseree S, Nalampang S. Age estimation using segmented Pulp/Tooth area ratio method in Thai population. J Dent Assoc Thai 2018; 68(2): 165-172.

26. Pitiphat W. Research methodology in dentisty. 1st ed. Khonkaen: Khonkaen university printing house; 2011:223.

27. Cohen J. Statistical power analysis for the behavioral sciences (2nd edn) 1988: 25-27.

28. Panyarak W. Oral radiograpy techniques for dental assistant [monograph on the internet]. Chiang mai: Faculty of dentistry, Chiang mai university; [cited 2017 Jan 25]. Available from: HYPERLINK

29. http://www.chiangmaihealth.go.th/cmpho_web/document/160429146191828718.pdf“http://www.chiangmaihealth.go.th/cmpho_web/document/160429146191828718.pdf”

30. Janhom A. Basic oral radiology. 1st ed. Chiang mai: Trio advertising & media co.,ltd; 2560:181.

Paleeta C, Janhom A, Prasitwattanaseree S. Age Estimation Using Pulp/Tooth Area Ratio of Upper Canines in a Thai Population: Original articles. CM Dent J [Internet]. 2019 Mar 20 [cited 2024 May 18];40(1):47-55. Available from: https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=77

Paleeta, C., Janhom, A. & Prasitwattanaseree, S. (2019). Age Estimation Using Pulp/Tooth Area Ratio of Upper Canines in a Thai Population. CM Dent J, 40(1), 47-55. Retrieved from: https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=77

Paleeta, C., Janhom Apirum and Prasitwattanaseree Sukon. 2019. "Age Estimation Using Pulp/Tooth Area Ratio of Upper Canines in a Thai Population." CM Dent J, 40(1), 47-55. https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=77

Paleeta, C. et al. 2019. 'Age Estimation Using Pulp/Tooth Area Ratio of Upper Canines in a Thai Population', CM Dent J, 40(1), 47-55. Retrieved from https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=77

Paleeta, C., Janhom, A. and Prasitwattanaseree, S. "Age Estimation Using Pulp/Tooth Area Ratio of Upper Canines in a Thai Population", CM Dent J, vol.40, no. 1, pp. 47-55, Mar. 2019.

Paleeta Chanunya, Janhom Apirum, Prasitwattanaseree Sukon "Age Estimation Using Pulp/Tooth Area Ratio of Upper Canines in a Thai Population." CM Dent J, vol.40, no. 1, Mar. 2019, pp. 47-55, https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=77