To date, chitosan-based hemostatic agents have gained increasing interest from their biocompatibility, inexpensiveness and hemostatic capability, especially in coagulopathic conditions. We have developed a functionally improved Calcium Alginate/N,O-carboxymethylchitosan (CA/NOCC) hemostatic sponge, shown to be biocompatible and biodegradable.
Objectives: To ensure its safe use with the gingival tissue, this study aimed to evaluate the cytotoxicity of CA/NOCC sponge on primary human gingival fibroblasts (GFs).
Methods: Human GFs were cultured with or without the CA/NOCC sponge. Cell morphology was assessed by scanning electron microscopy. Cell viability and proliferation were determined by MTT assays. The levels of Ca2+ released into the culture medium were also measured.
Results: Gingival fibroblasts cultured with the CA/NOCC sponge demonstrated lowered cell density, and significant ultrastructural changes of the cell membrane, by forming numerous blebs and fibrils. From MTT assays, approximately 30% decrease in the proliferation rate was observed. Moreover, the levels of Ca2+, up to 4.6 mM, were detected in the medium of GFs cultured with the CA/NOCC sponge.
Conclusions: It could be implicated that the cytopathic effects on the morphology and proliferative ability of GFs may result from the high level of Ca2+ released from the CA/NOCC hemostatic sponge.
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Mahatchariyapong T, Phaewpala K, Kosorn W, Kitrueangphatchara K, Sricholpech M. Cytotoxicity of the Calcium Alginate/N,O-carboxymethylchitosan Hemostatic Sponge on Primary Human Gingival Fibroblasts: Original articles. CM Dent J [Internet]. 2022 Jan 27 [cited 2024 Apr 29];43(1):19-28. Available from: https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=158
Mahatchariyapong, T., Phaewpala, K., Kosorn, W., Kitrueangphatchara, K. & Sricholpech, M. (2022). Cytotoxicity of the Calcium Alginate/N,O-carboxymethylchitosan Hemostatic Sponge on Primary Human Gingival Fibroblasts. CM Dent J, 43(1), 19-28. Retrieved from: https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=158
Mahatchariyapong, T., Phaewpala Kaltimas,Kosorn Wasana,Kitrueangphatchara Kitwat and Sricholpech Marnisa. 2022. "Cytotoxicity of the Calcium Alginate/N,O-carboxymethylchitosan Hemostatic Sponge on Primary Human Gingival Fibroblasts." CM Dent J, 43(1), 19-28. https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=158
Mahatchariyapong, T. et al. 2022. 'Cytotoxicity of the Calcium Alginate/N,O-carboxymethylchitosan Hemostatic Sponge on Primary Human Gingival Fibroblasts', CM Dent J, 43(1), 19-28. Retrieved from https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=158
Mahatchariyapong, T., Phaewpala, K., Kosorn, W., Kitrueangphatchara, K. and Sricholpech, M. "Cytotoxicity of the Calcium Alginate/N,O-carboxymethylchitosan Hemostatic Sponge on Primary Human Gingival Fibroblasts", CM Dent J, vol.43, no. 1, pp. 19-28, Jan. 2022.
Mahatchariyapong, T., Phaewpala, K., Kosorn, W., et al. "Cytotoxicity of the Calcium Alginate/N,O-carboxymethylchitosan Hemostatic Sponge on Primary Human Gingival Fibroblasts." CM Dent J, vol.43, no. 1, Jan. 2022, pp. 19-28, https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=158
