Biological Effects of Pulp Capping Material Containing Fluocinolone Acetonide on Human Dental Pulp Cells

Louwakul Phumisak , Krongbaramee Tadkamol, Louwakul Wilawan, Lertchirakarn Veera

Published Online: Sep 30, 2021 DOI 10.14456/cmdj.2021.9

Objectives: The aim of this study was to test some biological properties of pulp capping material containing fluocinolone acetonide (PCFA).

Materials and Methods: The PCFA is a hard setting calcium hydroxide cement containing 50 mmol/L of fluocinolone acetonide (FA). Cytotoxicity and cell proliferation were evaluated by MTT assay. RT-PCR and Western blotting were used to study the effects of PCFA on RNA (dentin sialophosphoprotein: DSPP) and protein (dentin sialoprotein: DSP) synthesis. Anti-inflammatory effect of PCFA was determined by analysis of cyclooxygenase 2 (COX-2) expression. Dycal® was used as control.

Results: The diluted conditioned media from PCFA slightly increased cell proliferation, significantly increased DSPP expression and decreased COX-2 expression (p < .05).

Conclusions: In term of biologic properties, the PCFA may promote cell proliferation, mineralization and decrease pre-existing inflammation in human dental pulp cells. It may be considered as an alternative pulp capping material in the treatment of inflamed dental pulp.

dental pulp fluocinolone acetonide inflammation in vitro

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Louwakul P, Krongbaramee T, Louwakul W, Lertchirakarn V. Biological Effects of Pulp Capping Material Containing Fluocinolone Acetonide on Human Dental Pulp Cells: Original articles. CM Dent J [Internet]. 2021 Sep 30 [cited 2024 May 02];42(3):85-94. Available from: https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=38

Louwakul, P., Krongbaramee, T., Louwakul, W. & Lertchirakarn, V. (2021). Biological Effects of Pulp Capping Material Containing Fluocinolone Acetonide on Human Dental Pulp Cells. CM Dent J, 42(3), 85-94. Retrieved from: https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=38

Louwakul, P., Krongbaramee Tadkamol,Louwakul Wilawan and Lertchirakarn Veera. 2021. "Biological Effects of Pulp Capping Material Containing Fluocinolone Acetonide on Human Dental Pulp Cells." CM Dent J, 42(3), 85-94. https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=38

Louwakul, P. et al. 2021. 'Biological Effects of Pulp Capping Material Containing Fluocinolone Acetonide on Human Dental Pulp Cells', CM Dent J, 42(3), 85-94. Retrieved from https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=38

Louwakul, P., Krongbaramee, T., Louwakul, W. and Lertchirakarn, V. "Biological Effects of Pulp Capping Material Containing Fluocinolone Acetonide on Human Dental Pulp Cells", CM Dent J, vol.42, no. 3, pp. 85-94, Sep. 2021.

Louwakul, P., Krongbaramee, T., Louwakul, W., et al. "Biological Effects of Pulp Capping Material Containing Fluocinolone Acetonide on Human Dental Pulp Cells." CM Dent J, vol.42, no. 3, Sep. 2021, pp. 85-94, https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=38