Regenerative Endodontic Therapy in a Necrotic Immature Tooth with Chronic Apical Abscess with Apical Placement of Calcium Hydroxide: A Clinical Case Report

Managing necrotic immature teeth is complex due to thin root walls and open apices. Regenerative endodontics has emerged to overcome these limitations. These biologically based approaches aim to restore pulp vitality and promote further root development by using dental stem cells and revascularization. A female patient aged 9 years and 4 months diagnosed with dens evaginatus, necrotic pulp, and a chronic apical abscess in an immature mandibular premolar underwent treatment with regenerative endodontic procedures (REPs). Calcium hydroxide (Ca(OH)2) medication was applied to the apical region of the root canal. Over a 22-month follow-up period, the tooth remained asymptomatic, and radiographic assessments revealed periapical healing, apical closure, root lengthening, and dentinal wall thickening. The favorable outcomes observed suggest that apical placement of Ca(OH)2 during REPs may be a promising approach for managing immature teeth with pulp necrosis and chronic apical abscess, supporting both disinfection and root maturation.

1. Mohammadi Z. Strategies to manage permanent nonvital teeth with open apices: a clinical update. Int Dent J. 2011;61(1):25-30.

2. Levitan ME, Himel VT. Dens evaginatus: literature review, pathophysiology, and comprehensive treatment regimen. J Endod. 2006;32(1):1-9.

3. Kocsis G, Marcsik A, Kókai E, Kocsis K. Supernumerary occlusal cusps on permanent human teeth. Acta Biol Szeged. 2002;46(1-2):71-82.

4. Lerdrungroj K, Banomyong D, Songtrakul K, Porkaew P, Nakornchai S. Current management of dens evaginatus teeth based on pulpal diagnosis. J Endod. 2023;49(10):1230-7.

5. Trope M. Treatment of the immature tooth with a nonvital pulp and apical periodontitis. Dent Clin North Am. 2010;54(2):313-24.

6. Wei X, Yang M, Yue L, Huang D, Zhou X, Wang X, et al. Expert consensus on regenerative endodontic procedures. Int J Oral Sci. 2022;14(1):55.

7. Bose R, Nummikoski P, Hargreaves K. A retrospective evaluation of radiographic outcomes in immature teeth with necrotic root canal systems treated with regenerative endodontic procedures. J Endod. 2009;35(10):1343-9.

8. Báez V, Corcos L, Morgillo F, Imperatrice L, Gualtieri AF. Meta-analysis of regenerative endodontics outcomes with antibiotics pastes and calcium hydroxide. the apex of the iceberg. J Oral Biol Craniofac Res. 2022;12(1):90-8.

9. Wigler R, Kaufman AY, Lin S, Steinbock N, HazanMolina H, Torneck CD. Revascularization: a treatment for permanent teeth with necrotic pulp and incomplete root development. J Endod. 2013;39(3):319-26.

10. Ajram J, Khalil I, Gergi R, Zogheib C. Management of an immature necrotic permanent molar with apical periodontitis treated by regenerative endodontic protocol using calcium hydroxide and MM-MTA: a case report with two years follow up. Dent J (Basel). 2019;7(1):1.

11. Park M, Ahn BD. Immature permanent teeth with apical periodontitis and abscess treated by regenerative endodontic treatment using calcium hydroxide and MTA: a report of two cases. Pediatr Dent. 2014;36(3):E107-10.

12. Chueh LH, Ho YC, Kuo TC, Lai WH, Chen YH, Chiang CP. Regenerative endodontic treatment for necrotic immature permanent teeth. J Endod. 2009;35(2):160–4.

13. Cehreli ZC, Isbitiren B, Sara S, Erbas G. Regenerative endodontic treatment (revascularization) of immature necrotic molars medicated with calcium hydroxide: a case series. J Endod. 2011;37(9):1327-30.

14. Pekpinarli B, Kaval ME, Cogulu D, Ilhan B, Sorsa T, Tervahartiala T, et al. The effect of calcium hydroxide and double antibiotic paste on radiographic outcomes and periapical MMP-8 levels in regenerative endodontic procedures: a randomized clinical trial. J Appl Oral Sci. 2024;32:e20240122.

15. Nagata JY, Gomes BP, Rocha Lima TF, Murakami LS, de Faria DE, Campos GR, et al. Traumatized immature teeth treated with 2 protocols of pulp revascularization. J Endod. 2014;40(5):606-12.

16. DeMayo FJ, Seagroves JT, Komabayashi T. Successful regenerative endodontic therapy of a dens evaginatus mandibular second premolar with an acute apical abscess and extensive periapical bone loss: a case report. Eur J Dent. 2025;19(1):255-64.

17. Cvek M. Prognosis of luxated non-vital maxillary incisors treated with calcium hydroxide and filled with gutta-percha. a retrospective clinical study. Endod Dent Traumatol. 1992;8(2):45-55.

18. American Association of Endodontists. Clinical considerations for a regenerative procedure [Internet]. Chicago: American Association of Endodontists; 2021 May 18 [updated 2021 May 18; cited 2025 Feb 3]. Available from: https://www.aae.org/specialty/wp-content/uploads/s... ConsiderationsApprovedByREC062921.pdf.

19. Silujjai J, Linsuwanont P. Treatment outcomes of apexification or revascularization in nonvital immature permanent teeth: a retrospective study. J Endod. 2017;43(2):238-45.

20. Estefan BS, El Batouty KM, Nagy MM, Diogenes A. Influence of age and apical diameter on the success of endodontic regeneration procedures. J Endod. 2016;42(11):1620-5.

21. Chueh LH, Huang GT. Immature teeth with periradicular periodontitis or abscess undergoing apexogenesis: a paradigm shift. J Endod. 2006;32(12):1205-13.

22. Kim YJ, Chandler NP. Determination of working length for teeth with wide or immature apices: a review. Int Endod J. 2013;46(6):483-91.

23. Mittal P, Jadhav GR, Logani A. Accuracy of different methods to determine working length in teeth with open apex-an ex-vivo comparative study. J Dent Specialities. 2016;4(1):39-45.

24. Haapasalo M, Shen Y, Wang Z, Gao Y. Irrigation in endodontics. Br Dent J. 2014;216(6):299 303.

25. Galler KM, Buchalla W, Hiller KA, Federlin M, Eidt A, Schiefersteiner M, et al. Influence of root canal disinfectants on growth factor release from dentin. J Endod. 2015;41(3):363-8.

26. Nguyen V. The purpose of calcium hydroxide in root canal treatment. IJRASET. 2023;11(3):433-4.

27. Safavi KE, Nichols FC. Effect of calcium hydroxide on bacterial lipopolysaccharide. J Endod. 1993;19(2):76-8.

28. Ruparel NB, Teixeira FB, Ferraz CC, Diogenes A. Direct effect of intracanal medicaments on survival of stem cells of the apical papilla. J Endod. 2012;38(10):1372-5.

29. Graham L, Cooper PR, Cassidy N, Nor JE, Sloan AJ, Smith AJ. The effect of calcium hydroxide on solubilisation of bio-active dentine matrix components. Biomaterials. 2006;27(14):2865-73.

30. Galler KM, Krastl G, Simon S, Van Gorp G, Meschi N, Vahedi B, et al. European Society of Endodontology position statement: revitalization procedures. Int Endod J. 2016;49(8):717-23.

31. Fava LR, Saunders WP. Calcium hydroxide pastes: classification and clinical indications. Int Endod J. 1999;32(4):257-82.

32. Stamos DG, Haasch GC, Gerstein H. The pH of local anesthetic/calcium hydroxide solutions. J Endod. 1985;11(6):264-5.

33. Ozcelik B, Taşman F, Oğan C. A comparison of the surface tension of calcium hydroxide mixed with different vehicles. J Endod. 2000;26(9):500-2.

34. Nelson SJ. Wheeler's Dental Anatomy, Physiology, &Occlusion. 10th ed. St. Louis: Elsevier; 2015.

35. Althumairy RI, Teixeira FB, Diogenes A. Effect of dentin conditioning with intracanal medicaments on survival of stem cells of apical papilla. J Endod. 2014;40(4):521-5.

36. Van Le H, Nguyen TA, Vu TS. Regenerative endodontic procedures of immature permanent premolars with periapical lesions: a report of two cases using two different materials, 18-month follow-up. Case Rep Dent. 2023;5577474.

37. de-Jesus-Soares A, Prado MC, Nardello LCL, Pereira AC, Cerqueira-Neto ACCL, Nagata JY, et al. Clinical and molecular microbiological evaluation of regenerative endodontic procedures in immature permanent teeth. J Endod. 2020;46(10):1448-54.

38. Mittal N, Baranwal HC, Kumar P, Gupta S. Assessment of pulp sensibility in the mature necrotic teeth using regenerative endodontic therapy with various scaffolds – randomized clinical trial. Indian J Dent Res. 2021;32(2):216-20.

39. Jiang X, Dai Y, Liu H. Evaluation of the characteristics of root canal calcification after regenerative endodontic procedures: a retrospective cohort study over 3 years. Int J Paediatr Dent. 2023;33(3):305-13.

40. Song M, Cao Y, Shin SJ, Shon WJ, Chugal N, Kim RH, et al. Revascularization-associated intracanal calcification: assessment of prevalence and contributing factors. J Endod. 2017;43(12):2025-33.

Saengmueankwan W, Sakulratchata R, Ruksakiet K, Puapichartdumrong P. Regenerative Endodontic Therapy in a Necrotic Immature Tooth with Chronic Apical Abscess with Apical Placement of Calcium Hydroxide: A Clinical Case Report: A case reports/series. Oral Sci Rep [Internet]. 2026 May 28 [cited 2026 May 28];47(2):272-278. Available from: https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=554

Saengmueankwan, W., Sakulratchata, R., Ruksakiet, K. & Puapichartdumrong, P. (2026). Regenerative Endodontic Therapy in a Necrotic Immature Tooth with Chronic Apical Abscess with Apical Placement of Calcium Hydroxide: A Clinical Case Report. Oral Sci Rep, 47(2), 272-278. Retrieved from: https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=554

Saengmueankwan, W., Ruedee Sakulratchata,Kasidid Ruksakiet and Peraya Puapichartdumrong. 2026. "Regenerative Endodontic Therapy in a Necrotic Immature Tooth with Chronic Apical Abscess with Apical Placement of Calcium Hydroxide: A Clinical Case Report." Oral Sci Rep, 47(2), 272-278. https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=554

Saengmueankwan, W. et al. 2026. 'Regenerative Endodontic Therapy in a Necrotic Immature Tooth with Chronic Apical Abscess with Apical Placement of Calcium Hydroxide: A Clinical Case Report', Oral Sci Rep, 47(2), 272-278. Retrieved from https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=554

Saengmueankwan, W., Sakulratchata, R., Ruksakiet, K. and Puapichartdumrong, P. "Regenerative Endodontic Therapy in a Necrotic Immature Tooth with Chronic Apical Abscess with Apical Placement of Calcium Hydroxide: A Clinical Case Report", Oral Sci Rep, vol.47, no. 2, pp. 272-278, May. 2026.

Saengmueankwan, W., Sakulratchata, R., Ruksakiet, K., et al. "Regenerative Endodontic Therapy in a Necrotic Immature Tooth with Chronic Apical Abscess with Apical Placement of Calcium Hydroxide: A Clinical Case Report." Oral Sci Rep, vol.47, no. 2, May. 2026, pp. 272-278, https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=554