Rapid Detection of Prevotella baroniae in Endodontic Infections
Objectives: Prevotella baroniae is an anaerobic bacterium that has recently been discovered and named. Identification of this new species is still difficult and time-consuming. The aims of this study were to generate and test a pair of specific primers for rapid detection of P. baroniae by polymerase chain reaction (PCR) and to investigate the incidence of this new candidate endodontic pathogen in endodontic infections in Thai population.
Methods: A pair of P. baroniae-specific primers was developed. The specificity and sensitivity of the primers were tested in PCR. Genomic DNA was isolated from samples collected from 56 patients who had endodontic infections and then the DNA was used as template in PCR. The presence of P. baroniae in the samples was investigated using the species-specific primers. The incidence of P. baroniae was then calculated and analyzed with Chi-Square test.
Results: P. baroniae-specific primers detected the target with no cross-reactivity. The specific fragment of approximately 848-bp was amplified only when the DNA of P. baroniae was used as template in the PCR reactions. Sensitivity testing showed that the estimated detection limit for the primers was 0.75 picogram of P. baroniae DNA. Overall, P. baroniae was detected in 12/56 (21.43%) of the samples. It was found in 6/24 (25.00%) of patients with acute symptoms and 6/32 (18.75%) of those with no symptoms. However, there was no significant difference between their occurrence and clinical symptoms.
Conclusions: Our results show that the PCR assay is effective for detecting P. baroniae from clinical specimens. Our findings confirm that this species is associated with endodontic infections. However, no significant association of this species with clinical symptoms was found.
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