Antibacterial Effect of Some Hemostatic Agents Used in Endodontics to Enterococcus faecalis and Bacteroides fragilis
Objectives: To study the antibacterial effects of some hemostatic agents to Enterococcus faecalis and Bacteroides fragilis.
Methods: Enterococcus faecalis strain JCM 7783 and Bacteroides fragilis strain ATCC 25285 were used to test the antibacterial properties of five hemostatic agents: epinephrine, aluminum chloride, aluminum sulfate, ferric sulfate and tranexamic acid; by using broth microdilution and disc diffusion tests. The positive control group was sodium hypochlorite and the negative control group was phosphate buffer saline.
Results: The results of broth microdilution test were reported in minimum inhibitory concentration (MIC) and minimum bactericidal concentrations (MBC) of aluminum chloride, aluminum sulfate and ferric sulfate to both Enterococcus faecalis and Bacteroides fragilis, which were 0.78125, 1.5625, 0.4845 percent, respectively. The disc diffusion test confirmed the antibacterial effects of aluminum chloride, aluminum sulfate and ferric sulfate by observation of the inhibition zones. Both epinephrine and tranexamic acid had no antibacterial activity.
Conclusions: Epinephrine and tranexamic acid has no antibacterial activity. Aluminum chloride, aluminum sulfate and ferric sulfate have antibacterial activity and may be beneficial for use as hemostatic agents in endodontic treatment.
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