Effect of Grape Seed Extract on Bond Durability to Sodium Hypochlorite-treated Dentin
Objective: To evaluate the effect of proanthocyanidin solution, which was extracted from grape seed, on microtensile bond strength and bond durability to sodium hypochlorite-treated dentin.
Methods: 48 human permanent molars were used in this study. A flat dentin surface was prepared by slicing horizontally parallel to the occlusal surface at 1 mm below the lowest level of the dentino-enamel junction. The specimens were divided into four groups. Group1, the dentin surface was rinsed with normal saline. Group 2, the dentin surface was treated with 5.25% sodium hypochlorite for 30 seconds. Groups 3 and 4, the dentin surface was treated with 5.25% sodium hypochlorite for 30 seconds, followed by 10% or 15% proanthocyanidin solution for 15 seconds, subsequently. All dentin specimens were rinsed with water and gently air dried, then were bonded with self-etch adhesive (Clearfill™ SE bond) following the manufacturer’s instructions and resin composite (Clearfil™ AP-X Esthetics) were built up. After 24 hours water storage, all specimens were cut into hour-glass shape, which had a bonded surface area of 1 mm2 approximately. Each group of specimens was divided into three subgroups by storage time; subgroup 1: microtensile bond strength was tested immediately; subgroups 2 and 3 were subjected to 2,500 and 5,000 cycles of thermocycling respectively. The microtensile bond strength of all bonded specimens was measured at a cross-head speed of 1 mm/min. All bond strength data were statistically analyzed using one-way ANOVA and Tamhane’s multiple comparison (p < 0.05)
Result: Considering the 24 hours water storage and 2,500 cycles of thermocycling subgroups, the mean value of microtensile bond strength in the groups treated only with sodium hypochlorite were significantly lower than those in all other groups. There were no significant difference between the groups treated with 10% and 15% proanthocyanidin solution and non-treated groups. However, after 5,000 cycles of thermocycling, there was no significant difference in the bond strength between all the treatment groups.
Conclusion: Using of 10% and 15% proanthocyanidin solution for 15 seconds on sodium hypochlorite-treated dentin could improve microtensile bond strength of self-etch adhesive after 24 hours storage time and bond durability after aging by thermocycling for 2,500 cycles.
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