The Study of Rice Gel as Bleaching Carrier on Microhardness and Roughness of Human Tooth

Objectives: The purpose of this study was to compare the effects of rice gel with bleaching agents and commercial bleaching gels on human tooth roughness and microhardness. The purpose of this study was to investigate the effects of rice gel with bleaching agents on human tooth roughness and microhardness compared with commercial bleaching gels.

Methods: Eighty four human tooth samples were prepared and randomly divided in to 7 groups (N=12). The samples were bleached with 7 gels as follow : 1) 10% rice gel with bleaching agents 2) 10% commercial bleaching gel 3) 20% rice gel with bleaching agents 4) 20% commercial bleaching gel 5) 35% commercial bleaching gel as a positive control 6) rice gel as a negative control 7) artificial saliva as a placebo. Surface roughness, Vicker’s microhardness and Scanning electron microscopy were tested. The average of surface roughness and Vicker’s microhardness were compared using the One-way ANOVA test (p<0.05).

Results: No significant differences in surface roughness and Vicker’s microhardness among the groups was found. For surface morphology of the commercial bleaching gels (Groups 2, 4 and 5), the enamel surface textures showed partially-etched surfaces with many shallow depressions, whereas the surface morphology of the rice gels with bleaching agents (Groups 1 and 3), the rice gel (Group 6) and the artificial saliva (Group 7) showed smooth and clear surfaces.

Conclusions: No Significant differences in surface roughness and Vicker’s microhardness on human tooth was found between rice gel with bleaching agents and commercial bleaching gels.

1. Setien VJ, Roshan S, Nelson PW. Clinical management of discolored teeth. Gen Dent 2008; 56: 294-300.

2. Giachetti L, Bertini F, Bambi C, et al. A randomized clinical trial comparing at-home and in-office tooth whitening techniques: A nine-month follow-up. J Am Dent Assoc 2010; 141: 1357-1364.

3. Auschill TM, Hellwig E, Schmidale S, et al. Efficacy, side-effects and patients' acceptance of different bleaching techniques (OTC, in-office, at-home). Oper Dent 2005; 30: 156-163.

4. Alqahtani MQ. Tooth-bleaching procedures and their controversial effects: A literature review. Saudi Dent J 2014; 26: 33-46.

5. Kwon SR, Wertz PW. Review of the mechanism of tooth whitening. J Esthet Restor Dent 2015; 27: 240-257.

6. Joiner A. The bleaching of teeth: a review of the literature. J Dent 2006; 34: 412-419.

7. Minoux M, Serfaty R. Vital tooth bleaching: biologic adverse effects-a review. Quintessence Int 2008; 39: 645-659.

8. Dahl JE, Pallesen U. Tooth bleaching a critical review of the biological aspects. Crit Rev Oral Biol Med 2003; 14: 292-304.

9. Mrzezo. Dental bleaching systems 2015 [updated 2015 Jan 31; cited 2018 Apr 1]. Available from: HYPERLINK"https://pocketdentistry.com/18-dental-bleaching-sy..." https://pocketdentistry.com/18-dental-bleaching-sy...

10. Rodrigues JA, Oliveira GP, Amaral CM. Effect of thickener agents on dental enamel microhardness submitted to at-home bleaching. Braz Oral Res 2007; 21: 170-175.

11. Al-Salehi SK, Wood DJ, Hatton PV. The effect of 24h non-stop hydrogen peroxide concentration on bovine enamel and dentine mineral content and microhardness. J Dent 2007; 35: 845-850.

12. Araujo O, Baratieri LN, Araujo E. In situ study of in-office bleaching procedures using light sources on human enamel microhardness. Oper Dent 2010; 35: 139-146.

13. Azer SS, Machado C, Sanchez E, et al. Effect of home bleaching systems on enamel nanohardness and elastic modulus. J Dent 2009; 37: 185-190.

14. Azrak B, Callaway A, Kurth P, et al. Influence of bleaching agents on surface roughness of sound or eroded dental enamel specimens. J Esthet Restor Dent 2010; 22: 391-399.

15. Efeoglu N, Wood D, Efeoglu C. Microcomputerised tomography evaluation of 10% carbamide peroxide applied to enamel. J Dent 2005; 33: 561-567.

16. Josey AL, Meyers IA, Romaniuk K, et al. The effect of a vital bleaching technique on enamel surface morphology and the bonding of composite resin to enamel. J Oral Rehabil 1996; 23: 244-250.

17. McGuckin RS, Babin JF, Meyer BJ. Alterations in human enamel surface morphology following vital bleaching. J Prosthet Dent 1992; 68: 754-760.

18. Bitter NC, Sanders JL. The effect of four bleaching agents on the enamel surface: a scanning electron microscopic study. Quintessence Int 1993; 24: 817-824.

19. Shannon H, Spencer P, Gross K, et al. Characterization of enamel exposed to 10% carbamide peroxide bleaching agents. Quintessence Int 1993; 24: 39-44.

20. Lewinstein I, Hirschfeld Z, Stabholz A, et al. Effect of hydrogen peroxide and sodium perborate on the microhardness of human enamel and dentin. J Endod 1994; 20: 61-63.

21. Zalkind M, Arwaz JR, Goldman A, et al. Surface morphology changes in human enamel, dentin and cementum following bleaching: a scanning electron microscopy study. Endod Dent Traumatol 1996; 12: 82-88.

22. Kwon YH, Huo MS, Kim KH, et al. Effects of hydrogen peroxide on the light reflectance and morphology of bovine enamel. J Oral Rehabil 2002; 29: 473-477.

23. Bailey SJ, Swift EJ. Effects of home bleaching products on composite resins. Quintessence Int 1992; 23: 489-494.

24. Kwon SR, Kurti SR, Oyoyo U, et al. Effect of various tooth whitening modalities on microhardness, surface roughness and surface morphology of the enamel. Odontology 2015; 103: 274-279.

25. Ernst CP, Marroquin BB, Willershausen-Zonnchen B. Effects of hydrogen peroxide-containing bleaching agents on the morphology of human enamel. Quintessence Int 1996; 27: 53-56.

26. Pretty IA, Edgar WM, Higham SM. The effect of bleaching on enamel susceptibility to acid erosion and demineralisation. Br Dent J 2005; 198: 285-290.

27. DE Abreu DR, Sasaki RT, Amaral FL, et al. Effect of home-use and in-office bleaching agents containing hydrogen peroxide associated with amorphous calcium phosphate on enamel microhardness and surface roughness. J Esthet Restor Dent 2011; 23: 158-168.

28. Dishman MV, Covey DA, Baughan LW. The effects of peroxide bleaching on composite to enamel bond strength. Dent Mater 1994; 10: 33-36.

29. Garcia-Godoy F, Dodge WW, Donohue M, et al. Composite resin bond strength after enamel bleaching. Oper Dent 1993; 18: 144-147.

30. Ruse ND, Smith DC, Torneck CD, et al. Preliminary surface analysis of etched, bleached, and normal bovine enamel. J Dent Res 1990; 69: 1610-1613.

31. Haywood VB, Leech T, Heymann HO, et al. Nightguard vital bleaching: effects on enamel surface texture and diffusion. Quintessence Int 1990; 21: 801-804.

32. Okonogi S, Khongkhunthian S, Jaturasitha S. Development of mucoadhesive buccal films from rice for pharmaceutical delivery systems. Drug Discov Ther 2014; 8: 262-267.

33. Okonogi S, Kaewpinta A, Khongkhunthian S, et al. Effect of rice variety on the physicochemical properties of the modified rice powders and their derived mucoadhesive gels. Drug Discov Ther 2015; 9: 221-228.

34. Okonogi S, Kaewpinta A, Yotsawimonwat S, et al. Preparation and characterization of lidocaine rice gel for oral application. Drug Discov Ther 2015; 9: 397-403.

35. Natthachai J, Marisa S, Chuanchom S, et al. In vitro effectiveness of rice gel as beaching carrier on color alteration of human tooth. CM Dent J 2018; 39: 59-69.(in Thai)

36. Lopes GC, Bonissoni L, Baratieri LN, et al. Effect of bleaching agents on the hardness and morphology of enamel. J Esthet Restor Dent 2002; 14: 24-30.

37. Mondelli RF, Azevedo JF, Francisconi PA, et al. Wear and surface roughness of bovine enamel submitted to bleaching. Eur J Esthet Dent 2009; 4: 396-403.

38. Tezel H, Ertas OS, Ozata F, et al. Effect of bleaching agents on calcium loss from the enamel surface. Quintessence Int 2007; 38: 339-347.

39. Attin T, Schmidlin PR, Wegehaupt F, et al. Influence of study design on the impact of bleaching agents on dental enamel microhardness: a review. Dent Mater 2009; 25: 143-157.

40. Sulieman M, Addy M, Macdonald E, et al. A safety study in vitro for the effects of an in-office bleaching system on the integrity of enamel and dentine. J Dent 2004; 32: 581-590.

41. Featherstone JD, O’Really MM, Shariati M, et al. Factors relating to demineralization and remineralization of the teeth. Oxford: S.A. Leach; 1986: 23-34.

42. Cadenaro M, Breschi L, Nucci C, et al. Effect of two in-office whitening agents on the enamel surface in vivo: a morphological and non-contact profilometric study. Oper Dent 2008; 33: 127-134.

43. Basting RT, Rodrigues AL, Serra MC. Micromorphology and surface roughness of sound and demineralized enamel and dentin bleached with a 10% carbamide peroxide bleaching agent. Am J Dent 2007; 20: 97-102.

44. Smidt A, Weller D, Roman I, et al. Effect of bleaching agents on microhardness and surface morphology of tooth enamel. Am J Dent 1998; 11: 83-85.

45. Murchison DF, Charlton DG, Moore BK. Carbamide peroxide bleaching: effects on enamel surface hardness and bonding. Oper Dent 1992; 17: 181-185.

46. Seghi RR, Denry I. Effects of external bleaching on indentation and abrasion characteristics of human enamel in vitro. J Dent Res 1992; 71: 1340-1344.

47. Potocnik I, Kosec L, Gaspersic D. Effect of 10% carbamide peroxide bleaching gel on enamel microhardness, microstructure, and mineral content. J Endod 2000; 26: 203-206.

48. Basting RT, Rodrigues AL, Jr., Serra MC. The effects of seven carbamide peroxide bleaching agents on enamel microhardness over time. J Am Dent Assoc 2003; 134: 1335-1342.

49. Chen HP, Chang CH, Liu JK, et al. Effect of fluoride containing bleaching agents on enamel surface properties. J Dent 2008; 36: 718-725.

50. Freitas P, Turssi C, Hara A, et al. Monitoring of demineralized dentin microhardness throughout and after bleaching. Am J Dent 2004; 17: 342-346.

51. Basting RT, Rodrigues JA, Serra MC, et al. Shear bond strength of enamel treated with seven carbamide peroxide bleaching agents. J Esthet Restor Dent 2004; 16: 250-259.

52. Joiner A. Review of the effects of peroxide on enamel and dentine properties. J Dent 2007; 35: 889-896.

53. Sa Y, Chen D, Liu Y, et al. Effects of two in-office bleaching agents with different pH values on enamel surface structure and color: an in situ vs. in vitro study. J Dent 2012; 40: 26-34.

54. Xu B, Li Q, Wang Y. Effects of pH values of hydrogen peroxide bleaching agents on enamel surface properties. Oper Dent 2011; 36: 554-562.

Kitisri N, Okonogi S, Khongkhunthian S, Chaijareenont P. The Study of Rice Gel as Bleaching Carrier on Microhardness and Roughness of Human Tooth: Original articles. CM Dent J [Internet]. 2020 Jan 01 [cited 2024 May 01];41(1):69-80. Available from: https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=44

Kitisri, N., Okonogi, S., Khongkhunthian, S. & Chaijareenont, P. (2020). The Study of Rice Gel as Bleaching Carrier on Microhardness and Roughness of Human Tooth. CM Dent J, 41(1), 69-80. Retrieved from: https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=44

Kitisri, N., Okonogi Siriporn,Khongkhunthian Sakornrat and Chaijareenont Pisaisit. 2020. "The Study of Rice Gel as Bleaching Carrier on Microhardness and Roughness of Human Tooth." CM Dent J, 41(1), 69-80. https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=44

Kitisri, N. et al. 2020. 'The Study of Rice Gel as Bleaching Carrier on Microhardness and Roughness of Human Tooth', CM Dent J, 41(1), 69-80. Retrieved from https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=44

Kitisri, N., Okonogi, S., Khongkhunthian, S. and Chaijareenont, P. "The Study of Rice Gel as Bleaching Carrier on Microhardness and Roughness of Human Tooth", CM Dent J, vol.41, no. 1, pp. 69-80, Jan. 2020.

Kitisri, N., Okonogi, S., Khongkhunthian, S., et al. "The Study of Rice Gel as Bleaching Carrier on Microhardness and Roughness of Human Tooth." CM Dent J, vol.41, no. 1, Jan. 2020, pp. 69-80, https://www.dent.cmu.ac.th/cmdj/frontend/web/?r=site/viewarticle&id=44